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DMEM = Dulbecco's Modified Eagle's Medium; <t>FOXA2</t> = forkhead box A2; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; H/R = hypoxia/reoxygenation; IgG = immunoglobulin G; NC = negative control; oe = overexpression; PAR4 = protease-activated receptor 4; RT-qPCR = reverse transcription quantitative polymerase chain reaction. ** p<0.01, *** p<0.001.
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DMEM = Dulbecco's Modified Eagle's Medium; FOXA2 = forkhead box A2; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; H/R = hypoxia/reoxygenation; IgG = immunoglobulin G; NC = negative control; oe = overexpression; PAR4 = protease-activated receptor 4; RT-qPCR = reverse transcription quantitative polymerase chain reaction. ** p<0.01, *** p<0.001.

Journal: Korean Circulation Journal

Article Title: UPF1 Alleviates Myocardial Ischemia-Reperfusion Injury by Regulating SMURF2 -Mediated Ubiquitination Degradation of FOXA2

doi: 10.4070/kcj.2024.0190

Figure Lengend Snippet: DMEM = Dulbecco's Modified Eagle's Medium; FOXA2 = forkhead box A2; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; H/R = hypoxia/reoxygenation; IgG = immunoglobulin G; NC = negative control; oe = overexpression; PAR4 = protease-activated receptor 4; RT-qPCR = reverse transcription quantitative polymerase chain reaction. ** p<0.01, *** p<0.001.

Article Snippet: After 1 hour of blockade with 5% bovine serum albumin, membranes were incubated with antibodies overnight at 4˚C: UPF1 (#12040, Cell Signaling Technology, CST, Danvers, MA, USA), FOXA2 (#8186T, 1:1,000, CST), PAR4 (#2328, 1:1,000, CST), Bax (ab32503, 1:1,000, Abcam, Cambridge, MA, USA), Cleaved caspase-3 (ab184787, 1:1,000, Abcam), SYVN1 (13473-1-AP, 1:1,000, Proteintech, Rosemont, IL, USA), SMURF2 (18038-1-AP, 1:1,000, Proteintech), WW domain containing E3 ubiquitin protein ligase 2 (WWP2; 12197-1-AP, 1:1,000, Proteintech).

Techniques: Modification, Negative Control, Over Expression, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

FOXA2 = forkhead box A2; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; H/R = hypoxia/reoxygenation; NC = negative control; SMURF2 = SMAD-specific E3 ubiquitin ligase 2. *** p<0.001.

Journal: Korean Circulation Journal

Article Title: UPF1 Alleviates Myocardial Ischemia-Reperfusion Injury by Regulating SMURF2 -Mediated Ubiquitination Degradation of FOXA2

doi: 10.4070/kcj.2024.0190

Figure Lengend Snippet: FOXA2 = forkhead box A2; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; H/R = hypoxia/reoxygenation; NC = negative control; SMURF2 = SMAD-specific E3 ubiquitin ligase 2. *** p<0.001.

Article Snippet: After 1 hour of blockade with 5% bovine serum albumin, membranes were incubated with antibodies overnight at 4˚C: UPF1 (#12040, Cell Signaling Technology, CST, Danvers, MA, USA), FOXA2 (#8186T, 1:1,000, CST), PAR4 (#2328, 1:1,000, CST), Bax (ab32503, 1:1,000, Abcam, Cambridge, MA, USA), Cleaved caspase-3 (ab184787, 1:1,000, Abcam), SYVN1 (13473-1-AP, 1:1,000, Proteintech, Rosemont, IL, USA), SMURF2 (18038-1-AP, 1:1,000, Proteintech), WW domain containing E3 ubiquitin protein ligase 2 (WWP2; 12197-1-AP, 1:1,000, Proteintech).

Techniques: Negative Control

GAPDH = glyceraldehyde 3-phosphate dehydrogenase; FOXA2 = forkhead box A2; I/R = ischemia/reperfusion; NC = negative control; oe = overexpression; PAR4 = protease-activated receptor 4; SMURF2 = SMAD-specific E3 ubiquitin ligase 2; TTC = tetrazolium chloride; UPF1 = up-frameshift 1. *** p<0.001.

Journal: Korean Circulation Journal

Article Title: UPF1 Alleviates Myocardial Ischemia-Reperfusion Injury by Regulating SMURF2 -Mediated Ubiquitination Degradation of FOXA2

doi: 10.4070/kcj.2024.0190

Figure Lengend Snippet: GAPDH = glyceraldehyde 3-phosphate dehydrogenase; FOXA2 = forkhead box A2; I/R = ischemia/reperfusion; NC = negative control; oe = overexpression; PAR4 = protease-activated receptor 4; SMURF2 = SMAD-specific E3 ubiquitin ligase 2; TTC = tetrazolium chloride; UPF1 = up-frameshift 1. *** p<0.001.

Article Snippet: After 1 hour of blockade with 5% bovine serum albumin, membranes were incubated with antibodies overnight at 4˚C: UPF1 (#12040, Cell Signaling Technology, CST, Danvers, MA, USA), FOXA2 (#8186T, 1:1,000, CST), PAR4 (#2328, 1:1,000, CST), Bax (ab32503, 1:1,000, Abcam, Cambridge, MA, USA), Cleaved caspase-3 (ab184787, 1:1,000, Abcam), SYVN1 (13473-1-AP, 1:1,000, Proteintech, Rosemont, IL, USA), SMURF2 (18038-1-AP, 1:1,000, Proteintech), WW domain containing E3 ubiquitin protein ligase 2 (WWP2; 12197-1-AP, 1:1,000, Proteintech).

Techniques: Negative Control, Over Expression

FOXA2 = forkhead box A2; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; H/R = hypoxia/reoxygenation; NC = negative control; oe = overexpression; PAR4 = protease-activated receptor 4; SMURF2 = SMAD-specific E3 ubiquitin ligase 2; UPF1 = up-frameshift 1. * p<0.05, ** p<0.01, *** p<0.001.

Journal: Korean Circulation Journal

Article Title: UPF1 Alleviates Myocardial Ischemia-Reperfusion Injury by Regulating SMURF2 -Mediated Ubiquitination Degradation of FOXA2

doi: 10.4070/kcj.2024.0190

Figure Lengend Snippet: FOXA2 = forkhead box A2; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; H/R = hypoxia/reoxygenation; NC = negative control; oe = overexpression; PAR4 = protease-activated receptor 4; SMURF2 = SMAD-specific E3 ubiquitin ligase 2; UPF1 = up-frameshift 1. * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: After 1 hour of blockade with 5% bovine serum albumin, membranes were incubated with antibodies overnight at 4˚C: UPF1 (#12040, Cell Signaling Technology, CST, Danvers, MA, USA), FOXA2 (#8186T, 1:1,000, CST), PAR4 (#2328, 1:1,000, CST), Bax (ab32503, 1:1,000, Abcam, Cambridge, MA, USA), Cleaved caspase-3 (ab184787, 1:1,000, Abcam), SYVN1 (13473-1-AP, 1:1,000, Proteintech, Rosemont, IL, USA), SMURF2 (18038-1-AP, 1:1,000, Proteintech), WW domain containing E3 ubiquitin protein ligase 2 (WWP2; 12197-1-AP, 1:1,000, Proteintech).

Techniques: Negative Control, Over Expression